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KMID : 1094720060110050377
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Biotechnology and Bioprocess Engineering
2006 Volume.11 No. 5 p.377 ~ p.381
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The effects of light intensity, inoculum size, and cell immobilisation on the treatment of sago effluent with Rhodopseudomonas palustris strain B1
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Ibrahim Shaliza
Vikineswary S.
Al-Azad Sujjat
Chong L. L.
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Abstract
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A study was carried out to determine a suitable light intensity and inoculum size for the growth ofRhodopseudomonas palustris strain B1. The pollution reduction of sago effluent using free and immobilisedR. palustris cells was also evaluated. The growth rate in glutamatemalate medium was highest at 4 klux compared to 2.5 and 3 klux. The optimal inoculum size was 10% (v/v). Both the COD and BOD of the sago effluent were reduced by 67% after three days of treatment. The difference in biomass production or BOD and COD removal with higher inoculum sizes of 15 and 20% was minimal. This could be attributed to limited nutrient availabillity in the substrate. The use of immobilised cells ofR. palustris reduced the pollution load 10% less compared to pollution reduction by free cells. Hence, there was no significant difference in using free or immobilised cells for the treatment of sago effluent.
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KEYWORD
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Rhodopseudomonas palustris, sago starch processing effluent, COD reduction, BOD reduction, agar immobilisation
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